Determination of Donnan equilibria by gel filtration
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چکیده
منابع مشابه
Gel filtration of sialoglycoproteins.
The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative g...
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Another method has been developed for obtaining a soluble apoprotein from the low density lipoprotein (LDL) of human plasma in the density class 1.019 < d < 1.063. The approximate molecular weight of the apoprotein subunit from this lipoprotein density class was determined by gel filtration on Sephadex G-200 to be about 80,000. Both on gel filtration and analytical ultracentrifugation the solub...
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Allen, R. J. L. (1940). Biochem. J. 34, 858. Bartlett, P. D., Grimmett, P., Beers, L. & Shelata, S. (1956). J. biol. Chem. 218, 419. Burton, K. (1956). Biochem. J. 62, 315. Caiger, P., Morton, R. K., Jarrett, I. G. & Filsell, 0. H. (1962). Biochem. J. 85, 351. Filsell, 0. H., Jarrett, I. G., Atkinson, M. R., Caiger, P. & Morton, R. K. (1963). Biochem. J. 89, 92. Fishkin, A. F. & Lata, G. F. (19...
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We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique. We have extended this strategy to th...
متن کاملDetermination of total urinary protein by combined gel filtration and automated biuret reaction.
Determination of total urinary protein is now more frequently requested of the clinical chemistry laboratory, one reason being the increased number of patients with renal transplants for whom total urinary protein is determined as an index of immunological rejection (1). Available manual methods are cumbersome and inefficient under clinical laboratory conditions. Concentrations for total urinar...
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ژورنال
عنوان ژورنال: Journal of Polymer Science: Polymer Chemistry Edition
سال: 1977
ISSN: 0360-6376,1542-9369
DOI: 10.1002/pol.1977.170150823